Members of the OPTI-FREE products (Express, Replenish, and Puremoist (Alcon, Fort Worth, TX, USA)) demonstrated significantly higher percentages of antimicrobial activity compared to the renu Advanced Formula (Bausch + Lomb, Rochester, NY, USA), Biotrue (Bausch + Lomb, Rochester, NY), Acuvue RevitaLens (Johnson & Johnson, Santa Ana, CA), and Lite products (Cooper Vision, Scottsville, NY), for four of the trophozoite strains tested. Acanthamoeba recovery plates were incubated for 14 days, after which positive wells were identified and cell concentrations determined using 50% endpoint method. Using standard methods of Acanthamoeba disinfection and quantification, Acanthamoeba ATCC 30461, 30868, 50370, and 50676 trophozoites were inoculated into each MPS and held for the manufacturer's recommended disinfection time. Here we compare the efficacy of seven major MPSs on the global market. Thus, it is critical that the Acanthamoeba disinfection efficacy of the popular MPSs be determined.
The patient's choice and use of multi-purpose solutions (MPSs) continue to be a risk factor for AK. AK is linked with contact lens use combined with noncompliance with contact lens care cleaning regimens. This investigation examines the effectiveness of several common contact lens solutions in the disinfection of Acanthamoeba, which causes a serious eye infection most often resulting from dysfunctional or improper use of contact lens products.Īcanthamoeba keratitis (AK) is an eye infection caused by a free-living amoeba, which can lead to extensive corneal damage and frequently blindness.
LENSCARE KOMBI PLUS PLUS
However, both Clear Care and Clear Care Plus demonstrated significantly greater disinfection efficacy against Acanthamoeba trophozoites and cysts, with and without lenses (P All preservative-free disinfection systems demonstrated similarly high rates of antimicrobial efficacy when challenged with bacteria or fungi, with or without lenses. All challenges were performed at the manufacturer’s recommended disinfection time. These same challenges were also conducted in the presence of the following contact lenses: Boston RGP, Acuvue Oasys, Biofinity, Ultra, and 2-week PremiO.
Stand-alone challenges using bacteria, fungi, and Acanthamoeba were prepared according to the International Standards Organization method 14729. These were the one-step (hydrogen peroxide-based) Clear Care and Clear Care Plus systems and the two-step (povidone-iodine-based) Cleadew system. PF disinfection systems were analyzed for antimicrobial efficacy. Therefore, the disinfection efficacy of preservative-free (PF) disinfection systems against bacteria, fungi, and Acanthamoeba trophozoites and cysts should be assessed as products with the most potential to be efficacious against resistant organisms. Importantly, some of these pathogens, such as Acanthamoeba, are particularly unsusceptible to biocides in common contact lens care solutions. ABSTRACT Microbial keratitis is a devastating disease that can cause eye damage and blindness and can be the result of infections by several common ocular pathogens.
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